Nirmala Parajuli, Ph.D.
DVM, Tribhuvan University, Nepal, 2001
MVSc, Seoul National University, S. Korea, 2005
Ph.D. Innsbruck Medical University, Austria, 2009
I am interested in unveiling the precise molecular mechanisms responsible for cold storage (CS)-induced renal damage. My research focuses on exploring novel mechanisms by which CS damages mitochondria and importantly, to identify a possible therapeutic target that could lead to improved mitochondrial function before and after transplantation. Specifically, I plan to investigate the role of proteasome in mediating mitochondrial damage and renal dysfunction after CS and transplantation. We have established a syngeneic rat kidney transplantation model in Dr. MacMillan-Crow’s laboratory(my post-doctoral mentor), which will be fundamental in studying my independent research project on proteasome mediated mitochondrial and renal damage. In addition, I’m also collaborating with Dr. MacMillan-Crow to study the molecular mechanisms that disrupt mitochondrial dynamics during renal CS and transplantation using our rat kidney transplant model.
Recent Research Support
163DG27600026 American Heart (PI) (07/01/16 – 06/30/19)
“Ubiquitin Proteasome System, Mitochondria and Renal Cold Storage”
5T32D61921 (Post-Doctoral Researcher) (07/01/13 – 06/01/16)
“Proteasome, mitochondira and CS/transplantation”
Shrum S, MacMillan-Crow LA, Parajuli, N. Cold storage exacerbates renal and mitochondrial dysfunction following transplantation. Journal of Kidney. 2(1), 2016 (PMID: 27066594).
Singh P, Parajuli, N, Mayeux PR, MacMillan-Crow LA. Renal mitochondrial lipid peroxidation during sepsis. Journal of Kidney. 2(1), 2016 (PMID: 27104220).
Patil N, Parajuli N, Mayeux PR, MacMillan-Crow LA. Inactivation of renal mitochondrial respiratory complexes and manganese superoxide dismutase during sepsis: mitochondria-targeted antioxidant mitigates injury even with delayed therapy. Am J Phsiol Renal Physiol. 306(7):F734-43, 2014 (PMID: 24500690).
Datta S, Parajuli N, Tymoszuk P, Ottina E, Parson W, Sgonc R, Villunger A, Doppler W. Replenishment of the B cell compartment after doxorubicin-induced hematopoietic toxicity is facilitated by STAT1. Journal of Leukocyte Biology. J Leukoc Biol. 95(6):853-66, 2014 (PMID: 24295829).
Hannesdóttir L, Tymoszuk P, Parajuli N, Wasmer MH, Philipp S, Daschil N, Datta S, Koller JB, Tripp CH, Stoitzner P, Müller-Holzner E, Wiegers GJ, Sexl V, Villunger A, Doppler W. Lapatinib and doxorubicin enhance the Stat1-dependent antitumor immune response. Eur J Immunol. 43(10):2718-29, 2013 (PMID: 23843024).
Marecki JC, Parajuli N, Crow JP, Macmillan-Crow LA. The Use of the Cre/loxP System to Study Oxidative Stress in Tissue-Specific Manganese Superoxide Dismutase Knockout Models. Antioxid Redox Signal. 20(10):1655-70, 2013 (PMID: 23843024).
Parajuli N and MacMillan-Crow LA. Role of reduced manganese superoxide dismutase in ischemia-reperfusion injury: a possible trigger for autophagy and mitochondrial biogenesis? Am J Physiol Renal Physiol. 304(3):F257-67, 2013 (PMID: 23195678).
Parajuli N, Campbell LH, Marine A, Brockbank KG, Macmillan-Crow LA. MitoQ blunts mitochondrial and renal damage during cold preservation of porcine kidneys. PLoS One. 7(11): e48590, 2012 (PMID: 23139796).
Parajuli N, Marine A, Simmons S, Saba H, Mitchell T, Shimizu T, Shirasawa T, Macmillan-Crow LA. Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout mouse. Free Radic Biol Med. 15;51(2):406-16, 2011 (PMID: 21571061).
Mitchell T, Saba H, Laakman J, Parajuli N, MacMillan-Crow LA. Role of mitochondrial-derived oxidants in renal tubular cell cold-storage injury. Free Radic Biol Med. 1;49(8):1273-82, 2010 (PMID: 20659553).