Nirmala Parajuli, DVM, Ph.D.

Nirmala Parajuli, Ph.D.Assistant Professor
Phone: (501) 364-4232
Laboratory (501) 364-2873
Email: Nparajuli@uams.edu

Education

DVM, Tribhuvan University, Nepal, 2001
MVSc, Seoul National University, S. Korea, 2005
Ph.D. Innsbruck Medical University, Austria, 2009

Research Interests

Long-term interest of my laboratory is to understand molecular mechanisms responsible for cold storage (CS)-induced renal damage. My research focuses on exploring novel mechanisms by which CS alters protein quality and renal function after transplantation, and importantly, to identify a possible therapeutic target that could lead to improved renal outcome after transplantation. Specifically, I plan to investigate the roles of proteasome, heat shock proteins and complement pathway during CS plus transplantation. We have established a rat kidney transplantation model, which will be fundamental in studying my independent research project on CS mediated renal damage. In addition, I’m also collaborating with Dr. MacMillan-Crow to study the molecular mechanisms that disrupt mitochondrial dynamics during renal CS and transplantation using our rat kidney transplant model.

Meet Dr. Parajuli’s Research Team

Recent Research Support

Current Research

Transformational Project Award (19TPA34850057 – American Heart Association (2019- 2022)
Targeting Renal Immunoproteasome during Cold Storage to Improve Transplant-Associated Cardiovascular Health”
Role: Principal Investigator

COBRE CTPR  (NIH- P20GM121293 (2018- 2020)
“Complements, Heat Shock Proteins, and Proteasome Function During Renal Transplantation”
PI: Alan Tackett
Role: Junior Investigator

Marion B. Lyon New Scientist Development Award (Arkansas Children’s Research Institute (2019- 2021)
“Targeting Immunoproteasome during Cold Storage to Improve Renal Transplant Outcome in Children”
Role: Principal Investigator

Medical Research Endowment (University of Arkansas for Medical Sciences(2019- 2020)
“Novel Role of Heat Shock Protein 72 during Renal Cold Storage plus Transplantation”
Role: Principal Investigator

Completed Research

Scientist Development Grant (SDG) – (16SDG27600026 -American Heart Association(2016-2019)
“Ubiquitin Proteasome System, Mitochondria and Renal Cold Storage”
Role:  Principal Investigator

Barton Award 17-DN-08( UAMS (2017-2018)
Novel Role of Immunoproteaseome during Renal Cold Storage and Transplantation”
Role: Principal Investigator

Fellow-to-Faculty Award (UAMS 2016-2017)
“Role of Ubiquitin Proteasome System in Renal”
Role: Principal Investigator

TRAINING PROGRAM IN THE PATHOPHYSIOLOGY OF RENAL DISEASE (T32DK61921( 2013- 2016)
Proteasome, mitochondria and cold storage/transplantation”
Principal Investigator: Sudhir Shah
Role: Trainee

NIH  (R43DK089659-01 (2010-2011)
“Assessment of MitoQ Therapy for Cold Preservation Renal Damage”
Principle Investigator: Lee Ann MacMillan-Crow
Role:  Co-Investigator

NIH/NIDDK (RO1DK078936-(2-NCE)(2008-2015)
“Manganese Superoxide Dismutase and Renal Ischemia/Reperfusion.”
Principle Investigator: Lee Ann MacMillan-Crow
Role: Co-Investigator

Publications

Parajuli N (2019). A cycle of altered proteasome and reactive oxygen species production in renal proximal tubular cells. Toxicol Forensic Med Open J. 4(1): 13-17. doi:17140/TFMOJ-4-128.

Tobacyk J, Parajuli N, Shrum S, MacMillan-Crow LA (2019). The first direct activity assay for the mitochondrial protease OMA1. Mitochondrion. 46:1-5. PMID: 30926535.

Lo S, MacMillan-Crow, Parajuli N (2018). Renal cold storage followed by transplantation impairs proteasome function and subsequently mitochondrial protein homeostasis. American Journal of Physiology Renal Physiology. doi: 10.1152/ajprenal.00316.2018. PMID: 30303714.

Parajuli N, Shrum S, Tobacyk J, Harb A, Arthur JM, MacMillan-Crow LA. Renal cold storage followed by transplantation impairs expression of key mitochondrial fission and fusion proteins. PLoS ONE 12(10): e0185542, 2017. PMID: 28977005

Shrum S, MacMillan-Crow LA, Parajuli, N. Cold storage exacerbates renal and mitochondrial dysfunction following transplantation. Journal of Kidney. 2(1), 2016. PMID: 27066594

Singh P, Parajuli, N, Mayeux PR, MacMillan-Crow LA. Renal mitochondrial lipid peroxidation during sepsis. Journal of Kidney. 2(1), 2016. PMID: 27104220

Patil N, Parajuli N, Mayeux PR, MacMillan-Crow LA. Inactivation of renal mitochondrial respiratory complexes and manganese superoxide dismutase during sepsis: mitochondria-targeted antioxidant mitigates injury even with delayed therapy. Am J Phsiol Renal Physiol. 306(7):F734-43, 2014. PMID: 24500690

Datta S, Parajuli N, Tymoszuk P, Ottina E, Parson W, Sgonc R, Villunger A, Doppler W. Replenishment of the B cell compartment after doxorubicin-induced hematopoietic toxicity is facilitated by STAT1. Journal of Leukocyte Biology. J Leukoc Biol. 95(6):853-66, 2014. PMID: 24295829

Hannesdóttir L, Tymoszuk P, Parajuli N, Wasmer MH, Philipp S, Daschil N, Datta S, Koller JB, Tripp CH, Stoitzner P, Müller-Holzner E, Wiegers GJ, Sexl V, Villunger A, Doppler W. Lapatinib and doxorubicin enhance the Stat1-dependent antitumor immune response. Eur J Immunol. 43(10):2718-29, 2013. PMID: 23843024

Marecki JC, Parajuli N, Crow JP, Macmillan-Crow LA. The Use of the Cre/loxP System to Study Oxidative Stress in Tissue-Specific Manganese Superoxide Dismutase Knockout Models. Antioxid Redox Signal. 20(10):1655-70, 2013. PMID: 23843024

Parajuli N and MacMillan-Crow LA. Role of reduced manganese superoxide dismutase in ischemia-reperfusion injury: a possible trigger for autophagy and mitochondrial biogenesis? Am J Physiol Renal Physiol. 304(3):F257-67, 2013. PMID: 23195678

Parajuli N, Campbell LH, Marine A, Brockbank KG, Macmillan-Crow LA. MitoQ blunts mitochondrial and renal damage during cold preservation of porcine kidneys. PLoS One. 7(11): e48590, 2012. PMID: 23139796

Parajuli N, Marine A, Simmons S, Saba H, Mitchell T, Shimizu T, Shirasawa T, Macmillan-Crow LA. Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout mouse. Free Radic Biol Med. 15;51(2):406-16, 2011. PMID: 21571061

Mitchell T, Saba H, Laakman J, Parajuli N, MacMillan-Crow LA. Role of mitochondrial-derived oxidants in renal tubular cell cold-storage injury. Free Radic Biol Med. 1;49(8):1273-82, 2010. PMID: 20659553

View Dr. Parajuli’s Publication List